Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis

Andrea M Cabibbe; Paolo Miotto; Raquel Moure; Fernando Alcaide; Silke Feuerriegel; Gianni Pozzi; Vladislav Nikolayevskyy; Francis Drobniewski; Stefan Niemann; Klaus Reither; Daniela M Cirillo; TM-REST Consortium; TB-CHILD Consortium

doi:10.1128/JCM.01824-15

Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis

J Clin Microbiol. 2015 Dec;53(12):3876-80. doi: 10.1128/JCM.01824-15. Epub 2015 Aug 5.

Collaborators

Patrizia Di Pietro, Floriana San Biagio, Enrico Alessi, Tony G Barbuzzi, Silva Tafaj, Elizabetha Bachiyska, Irina Kontsevaya, Yanina Balabanova, Elisa Lazzeri, Joseph Sserunkuma, Francesco Aloi, Martin Nsubuga, Mohamed Sasamalo

Affiliations

¹ Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.
² Servei de Microbiologia, Hospital Universitari de Bellvitge-IDIBELL, Universitat de Barcelona, Barcelona, Spain.
³ Molecular Mycobacteriology, Research Center Borstel, Borstel, Germany.
⁴ Laboratory of Molecular Microbiology and Biotechnology, Department of Medical Biotechnologies, University of Siena, Siena, Italy.
⁵ Clinical TB and HIV Group, Blizard Institute, Queen Mary University of London, London, United Kingdom Department of Infectious Diseases and Immunity, Imperial College London, London, United Kingdom.
⁶ Swiss Tropical and Public Health Institute, Basel, Switzerland Ifakara Health Institute, Bagamoyo, United Republic of Tanzania.
⁷ Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy cirillo.daniela@hsr.it.

Abstract

We evaluated the performance of the molecular lab-on-chip-based VerePLEX Biosystem for detection of multidrug-resistant tuberculosis (MDR-TB), obtaining a diagnostic accuracy of more than 97.8% compared to sequencing and MTBDRplus assay for Mycobacterium tuberculosis complex and rifampin and isoniazid resistance detection on clinical isolates and smear-positive specimens. The speed, user-friendly interface, and versatility make it suitable for routine laboratory use.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Antitubercular Agents / pharmacology
Drug Resistance, Multiple, Bacterial
Humans
Isoniazid / pharmacology
Lab-On-A-Chip Devices*
Molecular Diagnostic Techniques / methods*
Mycobacterium tuberculosis / drug effects
Rifampin / pharmacology
Tuberculosis, Multidrug-Resistant / diagnosis*

Substances

Antitubercular Agents
Isoniazid
Rifampin