Disposable silicon-based all-in-one micro-qPCR for rapid on-site detection of pathogens.
Nunez-Bajo E, Silva Pinto Collins A, Kasimatis M, Cotur Y, Asfour T, Tanriverdi U, Grell M, Kaisti M, Senesi G, Stevenson K, Güder F.
Nunez-Bajo E, et al.
Nat Commun. 2020 Dec 2;11(1):6176. doi: 10.1038/s41467-020-19911-6.
Nat Commun. 2020.
PMID: 33268779
Free PMC article.
We are able to quantitatively detect a 563 bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real-time PCR with a limit-of-detection of 20 fg, equivalent to a single bacterium, at the 35(th) cycle. ...
We are able to quantitatively detect a 563 bp fragment of genomic DNA of Mycobacterium avium subspecies paratuberculosis through real …