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Crystallization chaperone strategies for membrane proteins.
Lieberman RL, Culver JA, Entzminger KC, Pai JC, Maynard JA. Lieberman RL, et al. Methods. 2011 Dec;55(4):293-302. doi: 10.1016/j.ymeth.2011.08.004. Epub 2011 Aug 11. Methods. 2011. PMID: 21854852 Free PMC article. Review.
Increased Fab thermoresistance via VH-targeted directed evolution.
Entzminger KC, Johnson JL, Hyun J, Lieberman RL, Maynard JA. Entzminger KC, et al. Protein Eng Des Sel. 2015 Oct;28(10):365-77. doi: 10.1093/protein/gzv037. Epub 2015 Aug 16. Protein Eng Des Sel. 2015. PMID: 26283664 Free PMC article.
Structural and biophysical characterization of an epitope-specific engineered Fab fragment and complexation with membrane proteins: implications for co-crystallization.
Johnson JL, Entzminger KC, Hyun J, Kalyoncu S, Heaner DP Jr, Morales IA, Sheppard A, Gumbart JC, Maynard JA, Lieberman RL. Johnson JL, et al. Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):896-906. doi: 10.1107/S1399004715001856. Epub 2015 Mar 27. Acta Crystallogr D Biol Crystallogr. 2015. PMID: 25849400 Free PMC article.