cDNA cloning and expression of a soluble epoxide hydrolase from human liver

Arch Biochem Biophys. 1993 Aug 15;305(1):197-201. doi: 10.1006/abbi.1993.1411.

Abstract

We report the cloning and expression of a cDNA that encodes a soluble epoxide hydrolase from human liver. The 2101-base clone predicts a 554-residue protein (M(r) 62,640) with an apparently imperfect peroxisomal targeting signal of Ser-Lys-Met at the carboxy terminus. The cDNA was expressed in the baculovirus system in the Spodoptera frugiperda 21 cell line. The recombinant protein was similar to soluble epoxide hydrolase isolated from human liver in terms of molecular weight, hydrolytic activity, inhibition, and immunoreactivity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Animals
  • Baculoviridae / genetics
  • Base Sequence
  • Cell Line
  • Cloning, Molecular*
  • DNA / genetics*
  • DNA Probes
  • Epoxide Hydrolases / chemistry
  • Epoxide Hydrolases / genetics*
  • Female
  • Gene Expression*
  • Humans
  • Liver / enzymology*
  • Molecular Sequence Data
  • Moths
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism

Substances

  • DNA Probes
  • RNA, Messenger
  • Recombinant Proteins
  • DNA
  • Epoxide Hydrolases

Associated data

  • GENBANK/L05779