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It takes two to tango: The dance of the permease.
Kaback HR, Guan L. Kaback HR, et al. J Gen Physiol. 2019 Jul 1;151(7):878-886. doi: 10.1085/jgp.201912377. Epub 2019 May 30. J Gen Physiol. 2019. PMID: 31147449 Free PMC article. Review.
Engineered occluded apo-intermediate of LacY.
Smirnova I, Kasho V, Kaback HR. Smirnova I, et al. Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):12716-12721. doi: 10.1073/pnas.1816267115. Epub 2018 Nov 26. Proc Natl Acad Sci U S A. 2018. PMID: 30478058 Free PMC article.
An Asymmetric Conformational Change in LacY.
Smirnova I, Kasho V, Jiang X, Kaback HR. Smirnova I, et al. Biochemistry. 2017 Apr 4;56(13):1943-1950. doi: 10.1021/acs.biochem.7b00134. Epub 2017 Mar 23. Biochemistry. 2017. PMID: 28300394 Free PMC article.
pKa of Glu325 in LacY.
Grytsyk N, Sugihara J, Kaback HR, Hellwig P. Grytsyk N, et al. Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):1530-1535. doi: 10.1073/pnas.1621431114. Epub 2017 Feb 1. Proc Natl Acad Sci U S A. 2017. PMID: 28154138 Free PMC article.
Crystal Structure of a ligand-bound LacY-Nanobody Complex.
Kumar H, Finer-Moore JS, Jiang X, Smirnova I, Kasho V, Pardon E, Steyaert J, Kaback HR, Stroud RM. Kumar H, et al. Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):8769-8774. doi: 10.1073/pnas.1801774115. Epub 2018 Aug 14. Proc Natl Acad Sci U S A. 2018. PMID: 30108145 Free PMC article.
Role of Conserved Gly-Gly Pairs on the Periplasmic Side of LacY.
Jiang X, Andersson M, Chau BT, Wong LY, Villafuerte MK, Kaback HR. Jiang X, et al. Biochemistry. 2016 Aug 9;55(31):4326-32. doi: 10.1021/acs.biochem.6b00666. Epub 2016 Aug 1. Biochemistry. 2016. PMID: 27438891 Free PMC article.
Arg302 governs the pKa of Glu325 in LacY.
Grytsyk N, Santos Seiça AF, Sugihara J, Kaback HR, Hellwig P. Grytsyk N, et al. Proc Natl Acad Sci U S A. 2019 Mar 12;116(11):4934-4939. doi: 10.1073/pnas.1820744116. Epub 2019 Feb 21. Proc Natl Acad Sci U S A. 2019. PMID: 30792352 Free PMC article.
11 results