Altered substrate specificity in flavocytochrome b2: structural insights into the mechanism of L-lactate dehydrogenation.
Mowat CG, Wehenkel A, Green AJ, Walkinshaw MD, Reid GA, Chapman SK.
Mowat CG, et al.
Biochemistry. 2004 Jul 27;43(29):9519-26. doi: 10.1021/bi049263m.
Biochemistry. 2004.
PMID: 15260495
The crystal structure of the enzyme, with pyruvate bound at the active site, has been determined [Xia, Z.-X., and Mathews, F. S. (1990) J. Mol. Biol. 212, 837-863]. The authors indicate that the methyl group of pyruvate is in close contact with Ala198 and Leu230. ...In par …
The crystal structure of the enzyme, with pyruvate bound at the active site, has been determined [Xia, Z.-X., and Mathews, F. S. (199 …