Wang H - Search Results

1

Seamless site-directed mutagenesis in complex cloned DNA sequences using the RedEx method.

Luan J, Song C, Liu Y, He R, Guo R, Cui Q, Jiang C, Li X, Hao K, Stewart AF, Fu J, Zhang Y, Wang H. Luan J, et al. Among authors: wang h. Nat Protoc. 2024 Nov;19(11):3360-3388. doi: 10.1038/s41596-024-01016-9. Epub 2024 Jul 15. Nat Protoc. 2024. PMID: 39009664 Review.

2

RecET direct cloning and Redαβ recombineering of biosynthetic gene clusters, large operons or single genes for heterologous expression.

Wang H, Li Z, Jia R, Hou Y, Yin J, Bian X, Li A, Müller R, Stewart AF, Fu J, Zhang Y. Wang H, et al. Nat Protoc. 2016 Jul;11(7):1175-90. doi: 10.1038/nprot.2016.054. Epub 2016 Jun 2. Nat Protoc. 2016. PMID: 27254463

3

RedEx: a method for seamless DNA insertion and deletion in large multimodular polyketide synthase gene clusters.

Song C, Luan J, Li R, Jiang C, Hou Y, Cui Q, Cui T, Tan L, Ma Z, Tang YJ, Stewart AF, Fu J, Zhang Y, Wang H. Song C, et al. Among authors: wang h. Nucleic Acids Res. 2020 Dec 16;48(22):e130. doi: 10.1093/nar/gkaa956. Nucleic Acids Res. 2020. PMID: 33119745 Free PMC article.

4

Improved seamless mutagenesis by recombineering using ccdB for counterselection.

Wang H, Bian X, Xia L, Ding X, Müller R, Zhang Y, Fu J, Stewart AF. Wang H, et al. Nucleic Acids Res. 2014 Mar;42(5):e37. doi: 10.1093/nar/gkt1339. Epub 2013 Dec 24. Nucleic Acids Res. 2014. PMID: 24369425 Free PMC article.

5

Enhanced Heterologous Spinosad Production from a 79-kb Synthetic Multioperon Assembly.

Song C, Luan J, Cui Q, Duan Q, Li Z, Gao Y, Li R, Li A, Shen Y, Li Y, Stewart AF, Zhang Y, Fu J, Wang H. Song C, et al. Among authors: wang h. ACS Synth Biol. 2019 Jan 18;8(1):137-147. doi: 10.1021/acssynbio.8b00402. Epub 2019 Jan 9. ACS Synth Biol. 2019. PMID: 30590919

6

Improving spinosad production by tuning expressions of the forosamine methyltransferase and the forosaminyl transferase to reduce undesired less active byproducts in the heterologous host Streptomyces albus J1074.

Li X, Guo R, Luan J, Fu J, Zhang Y, Wang H. Li X, et al. Among authors: wang h. Microb Cell Fact. 2023 Jan 19;22(1):15. doi: 10.1186/s12934-023-02023-3. Microb Cell Fact. 2023. PMID: 36658647 Free PMC article.

7

Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting.

Fu J, Bian X, Hu S, Wang H, Huang F, Seibert PM, Plaza A, Xia L, Müller R, Stewart AF, Zhang Y. Fu J, et al. Among authors: wang h. Nat Biotechnol. 2012 May;30(5):440-6. doi: 10.1038/nbt.2183. Nat Biotechnol. 2012. PMID: 22544021

8

ExoCET: exonuclease in vitro assembly combined with RecET recombination for highly efficient direct DNA cloning from complex genomes.

Wang H, Li Z, Jia R, Yin J, Li A, Xia L, Yin Y, Müller R, Fu J, Stewart AF, Zhang Y. Wang H, et al. Nucleic Acids Res. 2018 Mar 16;46(5):e28. doi: 10.1093/nar/gkx1249. Nucleic Acids Res. 2018. PMID: 29240926 Free PMC article.

9

Recombineering for Genetic Engineering of Natural Product Biosynthetic Pathways.

Abbasi MN, Fu J, Bian X, Wang H, Zhang Y, Li A. Abbasi MN, et al. Among authors: wang h. Trends Biotechnol. 2020 Jul;38(7):715-728. doi: 10.1016/j.tibtech.2019.12.018. Epub 2020 Jan 20. Trends Biotechnol. 2020. PMID: 31973879 Review.

10

Establishing an efficient salinomycin biosynthetic pathway in three heterologous Streptomyces hosts by constructing a 106-kb multioperon artificial gene cluster.

Jiang C, Zhou H, Sun H, He R, Song C, Cui T, Luan J, Fu J, Zhang Y, Jiao N, Wang H. Jiang C, et al. Among authors: wang h. Biotechnol Bioeng. 2021 Dec;118(12):4668-4677. doi: 10.1002/bit.27928. Epub 2021 Sep 1. Biotechnol Bioeng. 2021. PMID: 34436784

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