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NmeCas9 is an intrinsically high-fidelity genome-editing platform.
Amrani N, Gao XD, Liu P, Edraki A, Mir A, Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG, Zhu LJ, Wolfe SA, Sontheimer EJ. Amrani N, et al. Among authors: liu p. Genome Biol. 2018 Dec 5;19(1):214. doi: 10.1186/s13059-018-1591-1. Genome Biol. 2018. PMID: 30518407 Free PMC article.
Enhanced Cas12a editing in mammalian cells and zebrafish.
Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazão JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA. Liu P, et al. Nucleic Acids Res. 2019 May 7;47(8):4169-4180. doi: 10.1093/nar/gkz184. Nucleic Acids Res. 2019. PMID: 30892626 Free PMC article.
Highly efficient therapeutic gene editing of human hematopoietic stem cells.
Wu Y, Zeng J, Roscoe BP, Liu P, Yao Q, Lazzarotto CR, Clement K, Cole MA, Luk K, Baricordi C, Shen AH, Ren C, Esrick EB, Manis JP, Dorfman DM, Williams DA, Biffi A, Brugnara C, Biasco L, Brendel C, Pinello L, Tsai SQ, Wolfe SA, Bauer DE. Wu Y, et al. Among authors: liu p. Nat Med. 2019 May;25(5):776-783. doi: 10.1038/s41591-019-0401-y. Epub 2019 Mar 25. Nat Med. 2019. PMID: 30911135 Free PMC article.
Genome-wide detection and analysis of CRISPR-Cas off-targets.
Rodríguez TC, Dadafarin S, Pratt HE, Liu P, Amrani N, Zhu LJ. Rodríguez TC, et al. Among authors: liu p. Prog Mol Biol Transl Sci. 2021;181:31-43. doi: 10.1016/bs.pmbts.2021.01.012. Epub 2021 Mar 4. Prog Mol Biol Transl Sci. 2021. PMID: 34127199
5'-Modifications improve potency and efficacy of DNA donors for precision genome editing.
Ghanta KS, Chen Z, Mir A, Dokshin GA, Krishnamurthy PM, Yoon Y, Gallant J, Xu P, Zhang XO, Ozturk AR, Shin M, Idrizi F, Liu P, Gneid H, Edraki A, Lawson ND, Rivera-Pérez JA, Sontheimer EJ, Watts JK, Mello CC. Ghanta KS, et al. Among authors: liu p. Elife. 2021 Oct 19;10:e72216. doi: 10.7554/eLife.72216. Elife. 2021. PMID: 34665130 Free PMC article.
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