Abstract
Putative peptide-synthetase-encoding DNA fragments were isolated from the Streptomyces coelicolor A3(2) chromosome using a PCR-based approach and mapped to a single approximately 35 kb segment. In integrative transformation experiments, DNA fragments from this region disrupted production of the calcium-dependent antibiotic (CDA) and had sequences characteristic of non-ribosomal peptide synthetases, thus proving that the cda locus had been cloned.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Chromosome Mapping
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Chromosomes, Bacterial*
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DNA Primers
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DNA, Bacterial / biosynthesis
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DNA, Bacterial / isolation & purification
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Genes, Bacterial*
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Ionophores / metabolism
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Molecular Weight
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Peptide Synthases / genetics*
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Peptide Synthases / metabolism
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Peptides
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Polymerase Chain Reaction
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Restriction Mapping
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Sequence Alignment
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Streptomyces / genetics*
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Streptomyces / metabolism*
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Transformation, Bacterial
Substances
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CDA antibiotic
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DNA Primers
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DNA, Bacterial
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Ionophores
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Peptides
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Peptide Synthases