Abstract
We have tested the reactivity of the monoclonal antibodies submitted to the ISOBM TD-4 Workshop for reactivity with a hybrid molecule consisting of the bacterial beta-galactosidase and most of the extracellular nonrepeat domain of MUC1/episialin. Two monoclonal antibodies submitted to the Workshop, 232A1 and M29, were directed against the protein moiety of this domain as shown by immunoblotting and immunoprecipitation.
MeSH terms
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Antibodies, Monoclonal / analysis*
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Antibody Specificity / immunology
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Escherichia coli / genetics
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Humans
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Immunoblotting
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Mucin-1 / immunology*
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Neoplasm Proteins / genetics
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Neoplasm Proteins / immunology*
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Precipitin Tests
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Recombinant Fusion Proteins
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Transfection
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beta-Galactosidase / genetics
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beta-Galactosidase / immunology
Substances
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Antibodies, Monoclonal
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Mucin-1
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Neoplasm Proteins
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Recombinant Fusion Proteins
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beta-Galactosidase