In vitro experimental studies of sialyl Lewis x and sialyl Lewis a on endothelial and carcinoma cells: crucial glycans on selectin ligands

Glycoconj J. 1997 Aug;14(5):593-600. doi: 10.1023/a:1018536509950.

Abstract

Extravasation from the blood of malignant tumour cells that form metastasis and leukocytes that go into tissues require contact between selectins and their sialyl Lewis x and sialyl Lewis a (sLe(x) and sLe(a) respectively) decorated ligands. Endothelial cells have been shown to express sLe(x) epitopes in lymph nodes and at sites of inflammation, and this is crucial for the selectin-dependent leukocyte traffic. Besides the ability to synthesize sLe(x) on sialylated N-acetyllactosamine via the action of alpha(1,3)fucosyltransferase(s), endothelial cells can also degrade sLe(x) to Lewis x through the action of alpha(2,3)sialidase(s). In addition, several epithelial tumors possess the machinery to synthesize sLe(x), which facilitates their adhesion to endothelial E- and P-selectin.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Endothelium, Vascular / physiology*
  • Fucosyltransferases / metabolism
  • Humans
  • Inflammation / physiopathology
  • Lymph Nodes / physiopathology
  • Molecular Sequence Data
  • Neoplasms / physiopathology*
  • Neuraminidase / metabolism
  • Oligosaccharides / biosynthesis*
  • Oligosaccharides / chemistry
  • Sialyl Lewis X Antigen

Substances

  • Oligosaccharides
  • Sialyl Lewis X Antigen
  • sialyl-Le(a) oligosaccharide
  • Fucosyltransferases
  • galactoside 3-fucosyltransferase
  • Neuraminidase