Abstract
Rat CL-6 is the most highly insulin-induced gene in a liver cell line and is expressed in proliferating liver during regeneration and development. CL-6 is now denoted INSIG1 (insulin-induced gene 1). Human INSIG1 was isolated and found to be 80% identical to the rat gene within the translated region. It was located on human chromosome 7 within band q36. The human INSIG1 promoter conferred a high level of expression in both liver and fibroblast cell lines. INSIG1 expression was upregulated at the transcriptional level in rat regenerating liver and induced in a model of murine adipocyte differentiation, suggesting that INSIG1 may play a role in growth and differentiation of tissues involved in metabolic control.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adipose Tissue / chemistry*
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Adipose Tissue / cytology
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Adipose Tissue / growth & development
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Differentiation / genetics
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Cell Differentiation / physiology
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Cells, Cultured
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Chromosome Mapping
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Chromosomes, Human, Pair 7 / genetics
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Cloning, Molecular
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Gene Expression / genetics
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Gene Expression Regulation / genetics
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Gene Expression Regulation / physiology
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Genes / genetics
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Genomic Library
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Humans
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Intracellular Signaling Peptides and Proteins
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Liver / chemistry*
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Liver / cytology
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Liver / growth & development
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Liver Regeneration / genetics
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Liver Regeneration / physiology
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Membrane Proteins*
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Molecular Sequence Data
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Promoter Regions, Genetic / physiology
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Proteins / genetics*
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Rats
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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INSIG1 protein, human
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Insig1 protein, rat
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Proteins