Nuclear Magnetic Resonance myocardial tagging is a potent non-invasive technique which enables the quantification of myocardial deformation, globally but also regionally at different time points during the cardiac cycle. By the use of presaturating pulses prior to the actual imaging sequence non-invasive markers or tags can be placed on the myocardium at end diastole, which move and deform with the underlying myocardium on which they were inscribed. Through combination of perpendicular sets of short- and long-axis images with tags, a three-dimensionally reconstructed left ventricle is obtained, subdivided in 32 myocardial cuboids for which the 3D coordinates of the corners are known at different time points in the cardiac cycle. From these data global and regional strains and quantitative measures of shape can be computed.