Abstract
We have identified a gene in Vibrio cholerae (epd) which encodes an erythrose-4-phosphate dehydrogenase activity and is located immediately downstream of an iron-regulated virulence gene, irgA, and immediately upstream of a gene encoding phosphoglycerate kinase (pgk). Expression of epd in V. cholerae is not regulated by iron, nor is it required for virulence in an infant mouse model.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aldehyde Oxidoreductases / genetics*
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Aldehyde Oxidoreductases / metabolism*
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Amino Acid Sequence
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Bacterial Proteins / genetics
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Base Sequence
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Cloning, Molecular
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Escherichia coli Proteins*
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Gene Expression Regulation, Bacterial / physiology
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Genes, Bacterial / genetics*
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Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
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Iron / physiology
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Molecular Sequence Data
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Phosphoglycerate Kinase / genetics
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Receptors, Cell Surface*
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Restriction Mapping
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Sequence Homology, Amino Acid
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Vibrio cholerae / enzymology
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Vibrio cholerae / genetics*
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Vibrio cholerae / pathogenicity
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Virulence
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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Receptors, Cell Surface
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irgA protein, Vibrio cholerae
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Iron
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Aldehyde Oxidoreductases
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Glyceraldehyde-3-Phosphate Dehydrogenases
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erythrose 4-phosphate dehydrogenase, E coli
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Phosphoglycerate Kinase