Abstract
Three monoclonal antibodies (MAbs) were generated from splenocytes of a BALB/c mouse immunized with heat-killed Mycobacterium tuberculosis. All three MAbs bound to surface epitopes of M. tuberculosis as shown by whole-cell enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence, and immunoelectron microscopy. One immunoglobulin M (IgM) MAb bound to lipoarabinomannan, the second IgM MAb bound to mycolyl-arabinogalactan-peptidoglycan complex, and the third MAb, an IgG3, bound to a surface epitope of an uncertain nature. The MAbs demonstrated different cross-reactivity patterns with other mycobacteria. Two of the MAbs were used to develop a modified ELISA spot assay for the detection of mycobacteria.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Bacterial*
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Antibodies, Monoclonal*
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Antigens, Bacterial
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Antigens, Surface
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Cross Reactions
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Enzyme-Linked Immunosorbent Assay / methods*
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Evaluation Studies as Topic
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Fluorescent Antibody Technique, Indirect
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Humans
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Hybridomas
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Mice
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Microscopy, Immunoelectron
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Mycobacterium / immunology*
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Mycobacterium / isolation & purification*
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Mycobacterium tuberculosis / immunology*
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Mycobacterium tuberculosis / isolation & purification*
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Mycobacterium tuberculosis / ultrastructure
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Species Specificity
Substances
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Antibodies, Bacterial
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Antibodies, Monoclonal
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Antigens, Bacterial
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Antigens, Surface