Evaluation of cyclohexenoesculetin-beta-D-galactoside and 8-hydroxyquinoline-beta-D-galactoside as substrates for the detection of beta-galactosidase

Appl Environ Microbiol. 1996 Oct;62(10):3868-70. doi: 10.1128/aem.62.10.3868-3870.1996.

Abstract

We describe the synthesis of two new substrates for the detection of beta-galactosidase and evaluate their performance in comparison with that of 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal). Of 171 Enterobacteriaceae strains that were able to hydrolyze X-Gal, 166 (97.1%) also hydrolyzed cyclohexenoesculetin-beta-D-galactoside whereas only 96 (56.1%) showed evidence of hydrolysis of 8-hydroxyquinoline-beta-D-galactoside. No false-positive results were observed with either substrate.

Publication types

  • Comparative Study

MeSH terms

  • Enterobacteriaceae / enzymology
  • Galactosides* / chemical synthesis
  • Galactosides* / metabolism
  • Hydrolysis
  • Hydroxyquinolines* / chemical synthesis
  • Hydroxyquinolines* / metabolism
  • Indicators and Reagents
  • Indoles / metabolism
  • Substrate Specificity
  • Umbelliferones* / chemical synthesis
  • Umbelliferones* / metabolism
  • beta-Galactosidase / analysis*

Substances

  • 8-hydroxyquinoline-beta-galactoside
  • Galactosides
  • Hydroxyquinolines
  • Indicators and Reagents
  • Indoles
  • Umbelliferones
  • cyclohexenoesculetin-beta-galactoside
  • beta-Galactosidase
  • 5-bromo-4-chloro-3-indolyl beta-galactoside