Simple method for detection of MYH11 DNA rearrangements in patients with inv(16)(p13q22) and acute myeloid leukemia

B A van der Reijden; D Martinet; J G Dauwerse; R H Giles; J W Wessels; G C Beverstock; B Smit; D Mühlematter; M Jotterand Bellomo; J Gabert; M Lafage-Pochitaloff; J Reiffers; C Bilhou-Nabera; G J van Ommen; A Hagemeijer; M H Breuning

Simple method for detection of MYH11 DNA rearrangements in patients with inv(16)(p13q22) and acute myeloid leukemia

Leukemia. 1996 Sep;10(9):1459-62.

Authors

B A van der Reijden¹, D Martinet, J G Dauwerse, R H Giles, J W Wessels, G C Beverstock, B Smit, D Mühlematter, M Jotterand Bellomo, J Gabert, M Lafage-Pochitaloff, J Reiffers, C Bilhou-Nabera, G J van Ommen, A Hagemeijer, M H Breuning

Affiliation

¹ Department of Human Genetics, Leiden University, Sylvius Laboratories, The Netherlands.

PMID: 8751463

Abstract

The pericentric inversion on chromosome 16 [inv(16)(p13q22)] and related t(16;16)(p13;q22) are recurrent aberrations associated with acute myeloid leukemia (AML) M4 Eo. Both abberations result in a fusion of the core binding factor beta (CBFB) and smooth muscle myosin heavy chain gene (MYH11). A selected genomic 6.9-kb BamHl probe detects MYH11 DNA rearrangements in 18 of 19 inv(16)/t(16;16) patients tested using HindIII digested DNA. The rearranged fragments were not detectable after remission in two cases tested, while they were present after relapse in one of these two cases tested.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Inversion*
Chromosomes, Human, Pair 16*
DNA, Neoplasm / genetics*
Gene Rearrangement*
Humans
Leukemia, Myelomonocytic, Acute / genetics*
Myosin Heavy Chains / biosynthesis
Myosin Heavy Chains / genetics*
Oncogene Proteins, Fusion / biosynthesis
Oncogene Proteins, Fusion / genetics
Translocation, Genetic

Substances

CBFbeta-MYH11 fusion protein
DNA, Neoplasm
Oncogene Proteins, Fusion
Myosin Heavy Chains