The feline herpesvirus type 1 ICP4 down-regulates feline immunodeficiency virus long terminal repeat (LTR)-directed gene expression via the C/EBP site in the LTR

J Vet Med Sci. 1995 Dec;57(6):1129-31. doi: 10.1292/jvms.57.1129.

Abstract

We investigated effects of feline herpesvirus type 1 (FHV-1) ICP4 on feline immunodeficiency virus (FIV) long terminal repeat (LTR)-directed gene expression by transient transfection assay in Crandell feline kidney cells. We demonstrated that FHV-1 ICP4 significantly stimulates the FIV LTR after introduction of site-specific mutation of the C/EBP site in the LTR, and the C/EBP site is sufficient to confer inhibitory effects by FHV-1 ICP4 on a heterologous promoter. These results indicate that FHV-1 ICP4 possesses both ability to transactivate FIV LTR-directed gene expression and to down-regulate the FIV LTR via the C/EBP site.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • CCAAT-Enhancer-Binding Proteins
  • Cats
  • Cell Line
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation, Viral*
  • Immediate-Early Proteins / biosynthesis
  • Immediate-Early Proteins / metabolism*
  • Immunodeficiency Virus, Feline / genetics*
  • Immunodeficiency Virus, Feline / metabolism
  • Kidney
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nuclear Proteins / metabolism*
  • Plasmids
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Repetitive Sequences, Nucleic Acid*
  • Simplexvirus*
  • Transcription Factors
  • Transfection

Substances

  • CCAAT-Enhancer-Binding Proteins
  • DNA-Binding Proteins
  • Immediate-Early Proteins
  • Nuclear Proteins
  • Recombinant Proteins
  • Transcription Factors
  • herpes simplex virus, type 1 protein ICP4