Urinary trypsinogen-2 test strip for acute pancreatitis

Lancet. 1996 Mar 16;347(9003):729-30. doi: 10.1016/s0140-6736(96)90078-1.

Abstract

Background: A simple, rapid test is specific and sensitive enough to distinguish, in patients with clinically suspected acute pancreatitis, those whose abdominal pain is indeed of pancreatic origin has proved elusive.

Methods: In two consecutive series of surgical patients in a teaching hospital, whose acute abdominal pain turned out to be due to acute pancreatitis (n-57) or extrapancreatic in origin (n=40), we studied urinary trypsinogen-2 in two ways. A test strip, incorporating monoclonal antibodies to two epitopes on trypsinogen-2, recorded a blue line when concentrations exceeded 50 microgram/L; we also measured trypsinogen-2 concentrations in the laboratory.

Findings: In the patients with acute pancreatitis the test strip was positive in 52 and negative in five, whereas in the 40 extrapancreatic controls there were four false positives. In a further set of 57 orthopaedic controls, one urine was strip-test positive. Concentrations of urinary trypsinogen-2 and the test-strip results were in good agreement and in only three of the 154 patients were the two approaches discrepant, at the 50 microgram/L cut-off.

Interpretation: These findings, in patients whose acute abdominal pain was known to be pancreatic in origin or not, are encouraging but need to be confirmed in a consecutive series of patients in whom the diagnosis of pancreatitis is in doubt.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abdominal Pain / diagnosis
  • Acute Disease
  • Adult
  • Aged
  • Aged, 80 and over
  • Diagnosis, Differential
  • False Positive Reactions
  • Female
  • Humans
  • Male
  • Middle Aged
  • Pancreatitis / diagnosis*
  • Reagent Strips*
  • Trypsin*
  • Trypsinogen / urine*

Substances

  • Reagent Strips
  • PRSS2 protein, human
  • Trypsinogen
  • Trypsin