Cloning and expression of a single-chain antibody fragment specific for a monomorphic determinant of class I molecules of the human major histocompatibility complex

Hybridoma. 1995 Oct;14(5):443-51. doi: 10.1089/hyb.1995.14.443.

Abstract

B9.12.1 is a monoclonal antibody specific for a monomorphic determinant of human MHC class I molecules. It is currently used for cell typing and is useful for targeting infection of human cells by murine ecotropic retroviruses. We have cloned and expressed it in the form of a single-chain variable fragment (ScFv) that recognizes the same epitope as the parental antibody. Through genetic engineering, this ScFv may be used for developing new cell-typing probes and new retroviral targeting approaches.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibody Specificity*
  • Base Sequence
  • Binding Sites, Antibody
  • Cloning, Molecular
  • Epitopes / chemistry
  • Epitopes / immunology*
  • Gene Expression / immunology
  • Genes, Immunoglobulin*
  • Genes, MHC Class I / immunology
  • Histocompatibility Antigens Class I / genetics*
  • Histocompatibility Antigens Class I / immunology*
  • Humans
  • Immunoglobulin Variable Region / biosynthesis
  • Immunoglobulin Variable Region / genetics*
  • Immunoglobulin Variable Region / isolation & purification
  • Molecular Sequence Data

Substances

  • Epitopes
  • Histocompatibility Antigens Class I
  • Immunoglobulin Variable Region