Magnetic bead capture of expressed sequences encoded within large genomic segments

Nature. 1993 Feb 25;361(6414):751-3. doi: 10.1038/361751a0.

Abstract

Magnetic bead capture utilizes biotin-streptavidin magnetic bead technology to isolate cDNAs rapidly from large genomic intervals, giving several thousand-fold enrichment of the selected cDNAs. The technique can allow parallel analysis of several large genomic segments of varying complexities and can be applied to the isolation of expressed sequences from various tissue sources.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins
  • Biotin
  • Chromosomes, Fungal
  • Cloning, Molecular / methods
  • Cosmids
  • DNA / genetics
  • DNA / isolation & purification*
  • DNA Probes
  • Gene Library
  • Genetic Techniques
  • Genome, Human*
  • Humans
  • Indicators and Reagents
  • Magnetics
  • Saccharomyces cerevisiae / genetics
  • Streptavidin

Substances

  • Bacterial Proteins
  • DNA Probes
  • Indicators and Reagents
  • Biotin
  • DNA
  • Streptavidin