Oligonucleotide DNA fingerprinting: results of a multi-center study on reliability and validity

EXS. 1993:67:257-60. doi: 10.1007/978-3-0348-8583-6_22.

Abstract

We report the results of an empirical study of 256 paternity cases referred to 7 different German laboratories for DNA fingerprinting with oligonucleotide probe (CAC)5/(GTG)5. All parameters characteristic of such multilocus DNA fingerprints were found to differ significantly between the contributing centres. Despite these differences, clear-cut decisions between paternity and non-paternity could be made in all but one case. Furthermore, we found no systematic deviation of the gel-phenotype distribution among trios from random expectation as derived from commonly adopted analytical models. Thus, we conclude that oligonucleotide DNA fingerprinting is a robust and reliable means for the resolution of paternity cases.

Publication types

  • Multicenter Study

MeSH terms

  • Base Sequence
  • DNA / genetics
  • DNA Fingerprinting / methods*
  • DNA Fingerprinting / statistics & numerical data
  • Evaluation Studies as Topic
  • Female
  • Humans
  • Male
  • Molecular Sequence Data
  • Oligonucleotide Probes / genetics
  • Paternity
  • Reproducibility of Results

Substances

  • Oligonucleotide Probes
  • DNA