The uptake of manganese dipyridoxal-diphosphate by chemically induced hepatocellular carcinoma in rats. A correlation between contrast-media-enhanced magnetic resonance imaging, tumor differentiation, and vascularization

Invest Radiol. 1993 Jun;28(6):520-8. doi: 10.1097/00004424-199306000-00011.

Abstract

Objectives and rationale: Negative enhancement of implanted liver tumors has been achieved in preclinical studies on manganese dipyridoxal-diphosphate (Mn-DPDP), a new hepatobiliary specific contrast agent for magnetic resonance imaging (MRI). The authors investigated the effects of Mn-DPDP on primary liver cancer and its possible mechanisms.

Methods: Magnetic resonance imaging was performed in 15 rats with chemically induced hepatocellular carcinoma (HCC) before and after Mn-DPDP injection. Both tumor-liver contrast-to-noise ratio and absolute tumor enhancement were evaluated and compared with the MRI results of a nonspecific contrast agent, gadolinium-DOTA, and correlated with corresponding microangiographic and histologic findings.

Results: Mn-DPDP injection led to a persistent positive enhancement in differentiated solid HCCs (22/23) with maximal conspicuity at 24 hours. Undifferentiated HCCs were all delineated by a prompt negative enhancement (20/20) with maximal conspicuity within 30 minutes.

MeSH terms

  • Angiography
  • Animals
  • Contrast Media*
  • Edetic Acid / analogs & derivatives*
  • Heterocyclic Compounds
  • Liver Neoplasms, Experimental / blood supply
  • Liver Neoplasms, Experimental / diagnosis*
  • Liver Neoplasms, Experimental / pathology*
  • Magnetic Resonance Imaging*
  • Male
  • Microradiography
  • Organometallic Compounds
  • Pyridoxal Phosphate / analogs & derivatives*
  • Rats
  • Rats, Wistar

Substances

  • Contrast Media
  • Heterocyclic Compounds
  • Organometallic Compounds
  • Pyridoxal Phosphate
  • gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate
  • Edetic Acid
  • N,N'-bis(pyridoxal-5-phosphate)ethylenediamine-N,N'-diacetic acid