Sequence and function analysis of a 4.3 kb fragment of Saccharomyces cerevisiae chromosome II including three open reading frames

Yeast. 1993 Aug;9(8):915-21. doi: 10.1002/yea.320090811.

Abstract

The nucleotide sequence of a fragment of 4337 base pairs of Saccharomyces cerevisiae chromosome II has been determined. The sequence contains three open reading frames, one of them being incomplete. Deletion analysis showed that YBR12.31 is essential for yeast growth, while deletion mutants of YBR12.32 and YBR12.33 are viable. YBR12.33 is identical to SMY2, isolated as a suppressor of a myo2 mutant (Lillie, S.H. and Brown, S.S., unpublished, EMBL M90654).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosomes, Fungal*
  • Codon / genetics
  • Fungal Proteins / genetics*
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Open Reading Frames / genetics*
  • Phenotype
  • Repressor Proteins / genetics*
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA
  • Sequence Deletion
  • Transformation, Genetic
  • Vesicular Transport Proteins

Substances

  • Codon
  • Fungal Proteins
  • Repressor Proteins
  • SMY2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Vesicular Transport Proteins

Associated data

  • GENBANK/M90654