Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata

M Krausová; M Klégr; J Spízek

doi:10.1007/BF02814521

Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata

Folia Microbiol (Praha). 1994;39(1):7-12. doi: 10.1007/BF02814521.

Authors

M Krausová¹, M Klégr, J Spízek

Affiliation

¹ Institute of Microbiology, Academy of Sciences of Czech Republic, Prague.

PMID: 8181783
DOI: 10.1007/BF02814521

Abstract

DNA-Dependent RNA polymerase (EC 2.7.7.6) was isolated from Thermomonospora curvata. The purification steps included precipitation with Polymin P, elution of the precipitate with 0.3 mol/L KCl, precipitation with ammonium sulfate, affinity chromatography on heparin-agarose and molecular filtration on Biogel A 1.5 m.

MeSH terms

Actinomycetales / enzymology*
Bacterial Proteins / isolation & purification*
Chemical Precipitation
Chromatography, Affinity
Chromatography, Gel
DNA-Directed RNA Polymerases / isolation & purification*
Molecular Weight
Spores, Bacterial

Substances

Bacterial Proteins
DNA-Directed RNA Polymerases