From May to June 1992, Apodemus agrarius and Apodemus speciosus were captured in Hunchun, Jilin Province. Four strains of Rickettsia tsutsugamushi were isolated from viscera of rats and trombiculid mites. At the same time, the antibody against Rickettsia tsutsugamushi was assayed in the sera of the local people and the wild rats. The positive rates were 15.2% and 16.4%, respectively. The above results showed that a natural focus of scrub typhus exists in Hunchun area.