Separation and identification of proteins obtained from Agkistrodon acutus snake venom by capillary zone electrophoresis and laser desorption/ionization mass monitoring

Y Zhang; N Chen; L Wang; B Yang; X Qian; L Ma

doi:10.1002/bmc.1130080312

Separation and identification of proteins obtained from Agkistrodon acutus snake venom by capillary zone electrophoresis and laser desorption/ionization mass monitoring

Biomed Chromatogr. 1994 May-Jun;8(3):148-50. doi: 10.1002/bmc.1130080312.

Authors

Y Zhang¹, N Chen, L Wang, B Yang, X Qian, L Ma

Affiliation

¹ National Chromatographic R. and A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences.

PMID: 8075526
DOI: 10.1002/bmc.1130080312

Abstract

Fractions of seven protein principles with fibrinolytic or thrombin-like activities obtained from Agkistrodon acutus snake venom purified by two steps of normal pressure chromatography were separated further by capillary zone electrophoresis (CZE). Mass determination for these fractions were achieved by performing laser desorption/ionization mass monitoring (LDIM). The comparative study between CZE and LDIM on the separation of these fractions was made.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agkistrodon*
Animals
Capillary Action
Electrophoresis / methods*
Lasers*
Mass Spectrometry*
Molecular Weight
Proteins / isolation & purification*
Snake Venoms / chemistry*

Substances

Proteins
Snake Venoms