The gene responsible for LEC hepatitis, located on rat chromosome 16, is the homolog to the human Wilson disease gene

Biochem Biophys Res Commun. 1994 Jul 15;202(1):512-8. doi: 10.1006/bbrc.1994.1958.

Abstract

We identified the rat homolog to the human Wilson disease (WD) gene as the gene responsible for hepatitis (hts) in the Long Evans Cinnamon (LEC) rat. A genetic study using fifty-three backcross progenies showed that the rat WD gene detected by Southern blotting using the human WD gene as a probe was tightly linked to the hts phenotype of the LEC rat with no recombination. LEC is a transcriptionally deficient mutant because no transcript of the rat WD gene could be found in the LEC rat by Northern blotting. This rat WD gene was mapped to 16q12.23-12.3 by fluorescence in situ hybridization and mouse x rat somatic cell hybrid analysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping*
  • Crosses, Genetic
  • DNA Primers
  • Female
  • Genomic Library
  • Hepatitis, Animal / genetics*
  • Hepatolenticular Degeneration / genetics*
  • Humans
  • Hybrid Cells
  • In Situ Hybridization, Fluorescence
  • Male
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Rats
  • Rats, Inbred Strains / genetics
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • DNA Primers