Expression of Marek's disease virus (MDV) serotype 2 gene which has partial homology with MDV serotype 1 pp38 gene

Virus Res. 1995 Feb;35(2):223-9. doi: 10.1016/0168-1702(94)00109-p.

Abstract

We constructed the recombinant baculovirus expressing the gene of non-pathogenic Marek's disease virus (MDV) serotype 2 (MDV2) which encodes a polypeptide with partial homology to MDV serotype 1 (MDV1) pp38, an antigen associated with transformed cells. The recombinant MDV2 protein was detected as a band of 32 kDa in immunoblot analysis with MDV2-infected chicken serum. Mouse serum against insect Spodoptera frugiperda cells infected with the recombinant baculovirus immunoprecipitated a 38 kDa molecule from the lysate of MDV2-infected chicken embryo fibroblasts (CEFs) but did not immunoprecipitate the MDV1 pp38 from the lysate of MDV1-infected CEFs. This result indicates that the recombinant MDV2 protein has no epitopes shared with the MDV1 pp38.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral / genetics*
  • Baculoviridae / genetics
  • Cell Line
  • Cloning, Molecular
  • DNA, Viral
  • Gene Expression
  • Genes, Viral*
  • Herpesvirus 2, Gallid / classification
  • Herpesvirus 2, Gallid / genetics*
  • Mice
  • Phosphoproteins / genetics*
  • Sequence Homology, Nucleic Acid
  • Serotyping
  • Spodoptera

Substances

  • Antigens, Viral
  • DNA, Viral
  • Phosphoproteins
  • phosphoprotein pp38, Marek's disease virus