Analysis of a novel cDNA encoding a C219-reactive peptide isolated from methotrexate-selected multidrug-resistant human leukemic cells

Gene. 1995 Apr 24;156(2):313-4. doi: 10.1016/0378-1119(94)00541-y.

Abstract

We have isolated and sequenced a novel cDNA clone (D320), from methotrexate-selected multidrug-resistant (MDR) human leukemic CCRF-CEM cells, which encodes a peptide reactive with monoclonal antibody (mAb) C219. Despite having been cloned using this ostensibly P-glycoprotein-specific mAb, clone D320 has no significant homology with either the MDR-encoding gene or, at the amino acid (aa) level, with its product, P-glycoprotein. A putative C219-binding site has been identified in D320, which differs at two positions from the 6-aa C219 epitope previously described in P-glycoprotein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / immunology*
  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Cross Reactions
  • DNA, Complementary / genetics
  • Drug Resistance, Multiple / genetics*
  • Drug Resistance, Multiple / immunology
  • Humans
  • Leukemia / genetics*
  • Methotrexate / pharmacology
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / immunology*
  • Recombinant Fusion Proteins / immunology
  • Selection, Genetic
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antibodies, Monoclonal
  • DNA, Complementary
  • Neoplasm Proteins
  • Recombinant Fusion Proteins
  • Methotrexate

Associated data

  • GENBANK/L34688