Anti-inflammatory effects of an adenosine kinase inhibitor. Decreased neutrophil accumulation and vascular leakage

J Immunol. 1995 May 15;154(10):5444-51.

Abstract

Adenosine inhibits neutrophil function, but also causes cardiovascular side effects when administered systemically. To regulate local adenosine concentrations and minimize toxicity, a novel adenosine kinase inhibitor, GP-1-515, was tested in several acute inflammation models in rats. GP-1-515 inhibited carrageenan-induced rat paw swelling in a dose-dependent manner (maximum inhibition, 47 +/- 3%). In a rat skin lesion model, GP-1-515 significantly reduced cutaneous neutrophil infiltration following an intradermal injection of carrageenan or zymosan-activated plasma, or induction of a reverse passive Arthus reaction. This action appeared to be mediated by endogenous adenosine, inasmuch as a specific A2 adenosine receptor antagonist reversed the effect. GP-1-515 also decreased vascular leakage induced by carrageenan (which is partly neutrophil dependent) and by the neutrophil-independent mediators histamine and bradykinin. Inhibition of leakage was reversed by co-administration of adenosine receptor antagonist. Treatment with anti-inflammatory doses of GP-1-515 had no effect on heart rate or blood pressure. In conclusion, GP-1-515 significantly reduced both neutrophil infiltration and vascular leakage through the release of endogenous adenosine without evidence of cardiovascular side effects.

MeSH terms

  • Adenosine Kinase / antagonists & inhibitors*
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Capillary Permeability / drug effects*
  • Carrageenan / antagonists & inhibitors
  • Chemotaxis, Leukocyte / drug effects*
  • Edema / pathology
  • Hemodynamics / drug effects
  • Male
  • Neutrophils / drug effects
  • Rats
  • Rats, Inbred Strains
  • Ribonucleosides / pharmacology*
  • Skin Diseases / pathology

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Ribonucleosides
  • GP 1-515
  • Carrageenan
  • Adenosine Kinase