Identification of T-cell epitopes using allele-specific ligand motifs

Behring Inst Mitt. 1994 Dec:(95):7-13.

Abstract

In order to facilitate the identification of T-cell epitopes as useful components of synthetic vaccines, we investigated the role of MHC molecules as the restriction element for the recognition of epitopes by the alpha beta receptor of T cells. MHC molecules are able to present thousands of different peptides to T cells, with all the peptides presented by one distinct type of MHC sharing common structural features. Our group analyzed these common characteristics concerning peptide length (only MHC I ligands) and anchor positions (MHC I and II ligands) occupied by a small set of closely related amino acids. Until now, for more than fifty MHC proteins allele-specific "peptide motifs" have been defined. The exact knowledge of MHC I peptide motifs allows for a prediction of CTL epitopes, and this kind of prediction has been successful in many cases over the last three years.

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • Epitopes / analysis*
  • HLA-D Antigens / immunology
  • Histocompatibility Antigens Class I / immunology
  • Histocompatibility Antigens Class II / immunology
  • Humans
  • Ligands
  • Molecular Sequence Data
  • Monophenol Monooxygenase / immunology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*

Substances

  • Epitopes
  • HLA-D Antigens
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Ligands
  • Monophenol Monooxygenase