Crystal structure of SIV matrix antigen and implications for virus assembly

Z Rao; A S Belyaev; E Fry; P Roy; I M Jones; D I Stuart

doi:10.1038/378743a0

Crystal structure of SIV matrix antigen and implications for virus assembly

Nature. 1995 Dec 14;378(6558):743-7. doi: 10.1038/378743a0.

Authors

Z Rao¹, A S Belyaev, E Fry, P Roy, I M Jones, D I Stuart

Affiliation

¹ Laboratory of Molecular Biophysics, Oxford, UK.

PMID: 7501025
DOI: 10.1038/378743a0

Abstract

Simian immunodeficiency virus (SIV) is closely related to human immunodeficiency virus (HIV), their matrix antigens (MAs) sharing some 50% sequence identity. MA is a component of Pr55Gag, the sole protein required for assembly of the virion shell. MA targets Pr55 to the plasma membrane, and facilitates incorporation of the virus envelope protein and assembly of the Pr55Gag shell. Cleavage of Pr55 by the viral protease produces the mature protein of relative molecular mass 17-18K, which underlies the host-derived membrane and is important in both virus entry and nuclear localization of the virion core. Here we report the crystal structure of SIV MA. The molecule forms a trimer consistent with oligomerization in vitro, the observed virion architecture, and various biological properties of MA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Antigens, Viral / chemistry*
Antigens, Viral / physiology
Computer Graphics
Crystallography, X-Ray
Gene Products, gag / chemistry*
Gene Products, gag / physiology
Models, Molecular
Molecular Sequence Data
Protein Conformation
Simian Immunodeficiency Virus / chemistry*
Simian Immunodeficiency Virus / physiology
Viral Matrix Proteins / chemistry*
Viral Matrix Proteins / physiology
Virus Replication

Substances

Antigens, Viral
Gene Products, gag
Viral Matrix Proteins
simian immunodeficiency virus matrix protein p17