Elevated EBF2 in mouse but not pig drives the progressive brown fat lineage specification via chromatin activation

Yinlong Liao; Zhelun Peng; Shanshan Fu; Yao Hua; Wenzhe Luo; Ruige Liu; Yingjin Chen; Wei Gu; Pengxiang Zhao; Jianguo Zhao; Yanfang Wang; Heng Wang

doi:10.1016/j.jare.2024.12.046

Elevated EBF2 in mouse but not pig drives the progressive brown fat lineage specification via chromatin activation

J Adv Res. 2024 Dec 28:S2090-1232(24)00624-6. doi: 10.1016/j.jare.2024.12.046. Online ahead of print.

Authors

Yinlong Liao¹, Zhelun Peng², Shanshan Fu³, Yao Hua³, Wenzhe Luo³, Ruige Liu⁴, Yingjin Chen⁴, Wei Gu⁵, Pengxiang Zhao³, Jianguo Zhao⁶, Yanfang Wang⁷, Heng Wang⁸

Affiliations

¹ College of Animal Science, Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, Shandong Agricultural University, Taian, China; Yazhouwan National Laboratory, Sanya, China.
² College of Animal Science, Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, Shandong Agricultural University, Taian, China; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
³ College of Animal Science, Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, Shandong Agricultural University, Taian, China.
⁴ College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
⁵ Shandong Provincial Key Laboratory of Animal Microecologics and Efficient Breeding of Livestock and Poultry, Shandong Baolai-Leelai Bio-Tech Co., Ltd, Taian, China.
⁶ Institute of Zoology, Chinese Academy of Science, Beijing, China.
⁷ Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
⁸ College of Animal Science, Shandong Provincial Key Laboratory for Livestock Germplasm Innovation & Utilization, Shandong Agricultural University, Taian, China; College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: wangheng@sdau.edu.cn.

PMID: 39736442
DOI: 10.1016/j.jare.2024.12.046

Abstract

Brown adipose tissue (BAT) is responsible for non-shivering thermogenesis, but it is absent in some mammals, including pigs. During development, BAT progenitors are derived from paired box 7 (Pax7)-expressing somitic mesodermal stem cells, which also give rise to skeletal muscle. However, the intrinsic mechanisms underlying the fate decisions between brown fat and muscle progenitors remain elusive. In this study, we analyzed the dynamics of chromatin landscape during the segregation of specification of brown fat and muscle lineages from Pax7⁺ progenitors, aiming to uncover epigenetic factors that drive BAT formation. Notably, myogenic progenitors were specified at embryonic day (E) 12.5, exhibiting high levels of H3K4me3 and low H3K27me3 at muscle-related genes. In contrast, the specification of the BAT lineage occurred much later, with coordinated step-wise depositions of histone modifications at BAT associated genes from E10.5 to E14.5. We identified the early B-cell factor 2 (EBF2) as a key driver of the progressive specification of brown fat lineage. Mechanistically, EBF2 interacts with transcriptional co-activators CREB binding protein/ E1A-binding protein p300 (CBP/P300) to induce chromatin acetylation at BAT-associated genes to promote brown adipogenesis. Both mouse and pig Ebf2 could potently stimulate adipogenesis in unspecified multipotent mesodermal stem cells. However, in pigs, EBF2 expression was depleted during the critical lineage specification time window, thus preventing the embryonic development of BAT. Hence, the elevation of EBF2 in mice, but not in pigs, promote chromatin activation to drive the progressive specification of brown fat lineage.

Keywords: Brown adipose tissue; Chromatin modification; Ebf2; Mouse; Pig.