Multi-responsive biosensor prepared based on MXene and PDEA-HRP binary architecture films for H2O2 detection and logic gate construction

Wenzheng Ma; Shuxue Zhen; Xiaoyan Shan; Ying Liu; Qiulong Liang; Changyi Yang; Runmei Ding; Lingchen Meng; Huiqin Yao

doi:10.1016/j.talanta.2024.127361

Multi-responsive biosensor prepared based on MXene and PDEA-HRP binary architecture films for H₂O₂ detection and logic gate construction

Talanta. 2024 Dec 13:285:127361. doi: 10.1016/j.talanta.2024.127361. Online ahead of print.

Authors

Wenzheng Ma¹, Shuxue Zhen¹, Xiaoyan Shan¹, Ying Liu¹, Qiulong Liang¹, Changyi Yang², Runmei Ding¹, Lingchen Meng³, Huiqin Yao⁴

Affiliations

¹ College of Public Health, School of Basic Medicine, Ningxia Medical University, Yinchuan, 750004, China.
² General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan, 750004, China.
³ College of Public Health, School of Basic Medicine, Ningxia Medical University, Yinchuan, 750004, China. Electronic address: 20140126@nxmu.edu.cn.
⁴ College of Public Health, School of Basic Medicine, Ningxia Medical University, Yinchuan, 750004, China. Electronic address: yaohq@nxmu.edu.cn.

PMID: 39700722
DOI: 10.1016/j.talanta.2024.127361

Abstract

The quantitative detection of H₂O₂ is of great significance for preventing the occurrence of diseases. In this work, an electrochemical biosensor for detecting H₂O₂ was constructed through a step-by-step modification method. The PDEA-HRP/MXene/PG biosensor (PDEA = poly(N,N-dimethyl acrylamide), HRP = horseradish peroxidase, PG = pyrolytic graphite) was prepared with two-dimensional metal carbide (MXene) nano materials as the inner layer and PDEA-HRP hydrogel as the outer layer for the detection of H₂O₂. Due to the excellent conductivity and biocompatibility of MXene materials, the prepared PDEA-HRP/MXene/PG biosensors have high sensitivity, wide linear range, and good repeatability. The results indicated that under optimal conditions, the prepared biosensor can detect H₂O₂ concentration within a linear range of 0.04 mM ∼ 1.80 mM, with the detection limit of 1.08 × 10^-3 mM (S/N = 3). The detection effect was good in actual samples. In addition, based on the switching properties of PDEA-HRP hydrogel under different conditions, combined with the characteristics of MXene nanomaterials. This study also constructed several biomolecule electrocatalytic logic gate systems, including binary 5-Input/5-Output logic gate network, 2-to-4 decoder, and a ternary AND logic gates.

Keywords: H(2)O(2); Horseradish peroxidase; Logic gate; MXene; Poly(N,N-diethylacrylamide).