Knockdown of HGH1 in breast cancer cell lines can inhibit the viability, invasion and migration of tumor cells

Zeyu Wang; Taiyuan Liu; Kang He; Longyun Wang; Xiaoxuan Ma; Zhaoyun Yang; Yingchao Zhang; Lijing Zhao

doi:10.1080/19336918.2024.2442349

Knockdown of HGH1 in breast cancer cell lines can inhibit the viability, invasion and migration of tumor cells

Cell Adh Migr. 2025 Dec;19(1):1-14. doi: 10.1080/19336918.2024.2442349. Epub 2024 Dec 18.

Authors

Zeyu Wang¹, Taiyuan Liu², Kang He³, Longyun Wang¹, Xiaoxuan Ma¹, Zhaoyun Yang¹, Yingchao Zhang², Lijing Zhao¹

Affiliations

¹ Department of Rehabilitation, School of Nursing, Jilin University, Changchun, China.
² Department of Breast Surgery, Second Hospital of Jilin University, Changchun, China.
³ Rehabilitation Medicine Center and Institute of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.

Abstract

Background: Research on the function of HGH1 in breast cancer remains lacking.

Methods: TCGAand GEO (GSE45827) datasets investigated discrepancies in HGH1 expression in BC. An aggregate of 106 clinical samples were gathered through immunohistochemistry, KM curves were drawn for prognostic analysis, and the function of HGH1 of BC was predicted. Finally, the effects of HGH1 knockdown on MDA-MB-231 and MCF-7 BC cells were verified via CCK8, invasion, wound healing and colony formation assays.

Results: HGH1 is highly expressed in BC and is linked to unfavorable prognosis. HGH1 overexpression is connected to keratinization and the cell cycle and is closely related to ER and PR expression and tumor stage in BC patients. Knocking down HGH1 in BC cells inhibited the viability, invasion and migration.

Conclusion: Knockdown of HGH1 in breast cancer cell lines can inhibit the viability, invasion and migration of tumor cells.

Keywords: Bioinformatics; HGH1; biomarker; breast cancer; prognosis.

MeSH terms

Breast Neoplasms* / genetics
Breast Neoplasms* / metabolism
Breast Neoplasms* / pathology
Cell Line, Tumor
Cell Movement* / genetics
Cell Proliferation
Cell Survival* / genetics
Female
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques*
Humans
MCF-7 Cells
Neoplasm Invasiveness*
Prognosis

Grants and funding

This study was supported by the Jilin Provincial Department of Science and Technology project (grant number: 20210204200YY) and the Healthcare Talents Program of the Finance Department of Jilin Province (grant number: 2024WSZX-B07).