Enhancing Crop Resilience: The Role of Plant Genetics, Transcription Factors, and Next-Generation Sequencing in Addressing Salt Stress

Int J Mol Sci. 2024 Nov 22;25(23):12537. doi: 10.3390/ijms252312537.

Abstract

Salt stress is a major abiotic stressor that limits plant growth, development, and agricultural productivity, especially in regions with high soil salinity. With the increasing salinization of soils due to climate change, developing salt-tolerant crops has become essential for ensuring food security. This review consolidates recent advances in plant genetics, transcription factors (TFs), and next-generation sequencing (NGS) technologies that are pivotal for enhancing salt stress tolerance in crops. It highlights critical genes involved in ion homeostasis, osmotic adjustment, and stress signaling pathways, which contribute to plant resilience under saline conditions. Additionally, specific TF families, such as DREB, NAC (NAM, ATAF, and CUC), and WRKY, are explored for their roles in activating salt-responsive gene networks. By leveraging NGS technologies-including genome-wide association studies (GWASs) and RNA sequencing (RNA-seq)-this review provides insights into the complex genetic basis of salt tolerance, identifying novel genes and regulatory networks that underpin adaptive responses. Emphasizing the integration of genetic tools, TF research, and NGS, this review presents a comprehensive framework for accelerating the development of salt-tolerant crops, contributing to sustainable agriculture in saline-prone areas.

Keywords: crop improvement; genetic transcription factors (TFs); next-generation sequencing (NGS); salt stress; salt tolerance.

Publication types

  • Review

MeSH terms

  • Crops, Agricultural* / genetics
  • Gene Expression Regulation, Plant
  • Gene Regulatory Networks
  • Genome-Wide Association Study / methods
  • High-Throughput Nucleotide Sequencing*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Salt Stress* / genetics
  • Salt Tolerance / genetics
  • Salt-Tolerant Plants / genetics
  • Transcription Factors* / genetics
  • Transcription Factors* / metabolism

Substances

  • Transcription Factors
  • Plant Proteins