Rice small protein OsS1Fa1 participates in stress responses as an inner nuclear membrane protein

Wang Ki Min; Kyu Ho Lee; Jong Tae Song; Hak Soo Seo

doi:10.1080/15592324.2024.2439252

Rice small protein OsS1Fa1 participates in stress responses as an inner nuclear membrane protein

Plant Signal Behav. 2024 Dec 31;19(1):2439252. doi: 10.1080/15592324.2024.2439252. Epub 2024 Dec 9.

Authors

Wang Ki Min¹, Kyu Ho Lee¹, Jong Tae Song², Hak Soo Seo^{1

3}

Affiliations

¹ Department of Agriculture, Forestry and Bioresources, Research Institute for Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul, Korea.
² Department of Applied Biosciences, Kyungpook National University, Daegu, Korea.
³ Bio-MAX Institute, Seoul National University, Seoul, Korea.

Abstract

The rice small protein OsS1Fa1, a homolog of spinach S1Fa, plays a significant role in drought tolerance, attributed to its transmembrane domain. In this study, we aim to further elucidate the potential roles of OsS1Fa1 in cold and biotic stresses as an inner nuclear membrane protein. Fluorescence analysis confirmed the localization of OsS1Fa1 to the inner nuclear membrane. Utilizing the bimolecular fluorescence complementation (BiFC) and bacterial infiltration assays with OsS1Fa1 and the inner nuclear membrane protein OsSUN1 (Rice Sad1 and UNC84 (SUN) domain containing 1 (SUN1)), we observed fluorescence detection within the inner nuclear membrane, indicating a direct interaction and colocalization between OsS1Fa1 and OsSUN1. Expression analysis revealed that overexpression of OsS1Fa1 induced the expression of various genes associated with cold and defense responses, including COLD-REGULATED 15A (COR15A), PATHOGENESIS-RELATED PROTEIN 1 (PR1), and PLANT DEFENSIN 1.2 (PDF1.2). Our findings collectively indicate that OsS1Fa1 plays crucial roles in both abiotic and biotic stress tolerance as an inner nuclear membrane protein.

Keywords: OsS1Fa1; OsSUN1; Rice; defense; inner nuclear membrane; stress.

MeSH terms

Gene Expression Regulation, Plant*
Membrane Proteins / genetics
Membrane Proteins / metabolism
Nuclear Envelope* / metabolism
Oryza* / genetics
Oryza* / metabolism
Plant Proteins* / genetics
Plant Proteins* / metabolism
Stress, Physiological* / genetics

Substances

Plant Proteins
Membrane Proteins

Grants and funding

This work was supported by National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIT) [Project No. 2021R1A2C1003446]. This work was also supported by Ascending SNU Future Leader Fellowship through Seoul National University.