Enhanced biosorption of cadmium ions on immobilized surface-engineered yeast using cadmium-binding peptides

Songting Wang; Yongmei Sun; Shihong Wang; Chunkun Fan; Daojie Wang; Fei Liu; Haiyan Zhang

doi:10.3389/fmicb.2024.1496843

Enhanced biosorption of cadmium ions on immobilized surface-engineered yeast using cadmium-binding peptides

Front Microbiol. 2024 Nov 15:15:1496843. doi: 10.3389/fmicb.2024.1496843. eCollection 2024.

Authors

Songting Wang^#^{1

2}, Yongmei Sun^#^{1

2}, Shihong Wang¹, Chunkun Fan³, Daojie Wang¹, Fei Liu¹, Haiyan Zhang^{1

2}

Affiliations

¹ School of Life Sciences, Henan University, Kaifeng, China.
² Engineering Research Center for Applied Microbiology of Henan Province, Kaifeng, China.
³ Institute of Agriculture Research, Tibet Academy of Agricultural and Animal Husbandry Science, Tibet, China.

^# Contributed equally.

Abstract

A new type of cadmium (Cd) ion cell surface adsorbent was developed by integrating bacteriophage display peptide library technology with cell surface display technology. Cd²⁺ chelating resin served as the target molecule in screening experiments, leading to the identification of four Cd²⁺ -binding peptides. These peptides were introduced into Saccharomyces cerevisiae via the pYD1 plasmid using lithium acetate heat shock transformation. Adsorption efficiency tests indicated that the engineered yeasts adsorbed more Cd²⁺ than the control strain EBY100 when exposed to the same amount of Cd²⁺. Among these peptides, sequence 3-containing strain was demonstrated to have the highest Cd²⁺ adsorption efficiency, being 35% higher than the control strain. Additionally, when this recombinant yeast strain was immobilized using sodium alginate, the adsorption efficiency was increased by 55.7% compared to the control strain.

Keywords: biosorption; cadmium-binding peptide; immobilization; phage peptide library; surface-engineered yeast.