Protective role of polysaccharide from the roots of Millettia speciosa Champ in mice with ulcerative colitis induced by dextran sulphate sodium

Yanfei Lu; Rihui Wu; Jiaquan Liu; Jinwen Huang; Qiu Liu; Min Yao; Jingyu Wu; Jiming Ye; Dongli Li; Lishe Gan; Jingwei Jin

doi:10.1080/14786419.2024.2435535

Protective role of polysaccharide from the roots of Millettia speciosa Champ in mice with ulcerative colitis induced by dextran sulphate sodium

Nat Prod Res. 2024 Dec 1:1-7. doi: 10.1080/14786419.2024.2435535. Online ahead of print.

Authors

Yanfei Lu^{1

2}, Rihui Wu^{1

2}, Jiaquan Liu^{1

2}, Jinwen Huang^{1

2}, Qiu Liu^{1

2}, Min Yao^{1

2}, Jingyu Wu^{1

2}, Jiming Ye³, Dongli Li^{1

2}, Lishe Gan^{1

2

4}, Jingwei Jin^{1

2}

Affiliations

¹ School of Pharmacy and Food Engineering, Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Wuyi University, Jiangmen, PR China.
² International Healthcare Innovation Institute (Jiangmen), Jiangmen, PR China.
³ Lipid Biology and Metabolic Disease Research Group, School of Health and Biomedical Sciences, RMIT University, Melbourne, Australia.
⁴ College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, PR China.

PMID: 39618179
DOI: 10.1080/14786419.2024.2435535

Abstract

This study investigated the therapeutic mechanism of Millettia Speciosa Champ. polysaccharide (MSCP), also known as Niu Dali polysaccharide, in treating ulcerative colitis (UC) using a dextran sulphate sodium (DSS) induced colitis model in C57BL/6 mice. Mice were randomly assigned to four groups: control, DSS model, and low- and high-dose MSCP treatment groups (50, 100 mg/kg/day, respectively). MSCP administration began concurrently with DSS induction. Throughout the intervention, changes in body weight and disease activity index were monitored. On day 44, colonic tissues were collected for analysis after measuring their lengths. Results demonstrated that MSCP effectively ameliorated DSS-induced chronic colitis by suppressing proinflammatory cytokine secretion, enhancing anti-inflammatory cytokine production, restoring intestinal epithelial barrier integrity, and downregulating TLR4 expression.

Keywords: Millettia speciosa champ; Polysaccharide conjugate; TLR4; Ulcerative colitis; intestinal barrier.