Lactobacillus reuteri metabolites alleviate apple replant disease (ARD) by driving beneficial bacteria to reshape the core root microbiome

Jinhui Lv; Xiaoxuan Li; Lei Zhao; Susu Zhang; Gongshuai Wang; Xiaoqi Wang; Yanfang Wang; Xuesen Chen; Chengmiao Yin; Zhiquan Mao

doi:10.1016/j.plaphy.2024.109345

Lactobacillus reuteri metabolites alleviate apple replant disease (ARD) by driving beneficial bacteria to reshape the core root microbiome

Plant Physiol Biochem. 2024 Nov 26:219:109345. doi: 10.1016/j.plaphy.2024.109345. Online ahead of print.

Authors

Jinhui Lv¹, Xiaoxuan Li¹, Lei Zhao¹, Susu Zhang², Gongshuai Wang², Xiaoqi Wang¹, Yanfang Wang³, Xuesen Chen¹, Chengmiao Yin⁴, Zhiquan Mao⁵

Affiliations

¹ College of Horticulture Science and Engineering Shandong Agricultural University, Tai'an, Shandong, 271018, PR China; Apple Technology Innovation Center of Shandong Province, Tai'an, Shandong, 271018, PR China.
² College of Forestry Engineering Shandong Agriculture and Engineering University, Ji'nan, Shandong, 250000, PR China.
³ College of Chemistry and Material Science Shandong Agricultural University, Tai'an, Shandong, 271018, PR China.
⁴ College of Horticulture Science and Engineering Shandong Agricultural University, Tai'an, Shandong, 271018, PR China; Apple Technology Innovation Center of Shandong Province, Tai'an, Shandong, 271018, PR China. Electronic address: yinchengmiao@163.com.
⁵ College of Horticulture Science and Engineering Shandong Agricultural University, Tai'an, Shandong, 271018, PR China; Apple Technology Innovation Center of Shandong Province, Tai'an, Shandong, 271018, PR China. Electronic address: mzhiquan@sdau.edu.

PMID: 39615192
DOI: 10.1016/j.plaphy.2024.109345

Abstract

Previous studies have shown that the bacterial fertilizer Lactobacillus reuteri (LBR) significantly alleviates apple replant disease (ARD), but the mechanism behind its effectiveness remains unclear. This study investigated the effects of key LBR metabolites on the rhizosphere microbial community. The biocontrol function of extracellular polysaccharides (EPS) was examined and shown to be further enhanced after optimizing the fermentation conditions. The optimized fermentation conditions were found to generate intermediates involved in various plant metabolic pathways, leading to plant growth promotion, increased abundance of beneficial bacteria like Bacillus and Pseudomonas in the rhizosphere soil, and decreased abundance of pathogenic fungi. Through the isolation and identification of rhizosphere microorganisms, a strain of Pseudomonas monteilii with chemotaxis to EPS was isolated, which had growth promotion ability and effectively improved plant resistance and relieves ARD. To further understand the mechanism underlying the inhibitory effect on soil pathogens of microbial aggregations and development in the rhizosphere driven by beneficial bacteria metabolites. These findings offer valuable technical insights for utilizing biocontrol bacteria metabolites in ARD management.

Keywords: Apple replant disease; Extracellular polysaccharide; Metabolite of Lactobacillus reuteri; Optimization of fermentation conditions; Pseudomonas monteilii; Rhizosphere microbial recruitment.