Osteogenesis promotion on MC3T3 by micro-area potential difference (MAPD) on titanium alloy

Yanchun Xie; Junfan Chen; Shan Fu; Hai-Long Yu; Anwu Xuan; Yongcun Wei; Yi Lian; Lei Yang; Erlin Zhang

doi:10.1088/1748-605X/ad98d7

Osteogenesis promotion on MC3T3 by micro-area potential difference (MAPD) on titanium alloy

Biomed Mater. 2024 Nov 29. doi: 10.1088/1748-605X/ad98d7. Online ahead of print.

Authors

Yanchun Xie¹, Junfan Chen², Shan Fu³, Hai-Long Yu⁴, Anwu Xuan¹, Yongcun Wei⁵, Yi Lian⁶, Lei Yang⁷, Erlin Zhang⁸

Affiliations

¹ General Hospital of Northern Theater Command, Shenyang 110016, China, Shenyang, Liaoning, 110016, CHINA.
² Northeastern University, Shenyang 110819, China, Shenyang, 110819, CHINA.
³ Northeastern University, Dalian 116051,China, Shenyang, Liaoning, 110819, CHINA.
⁴ Dalian Medical University, Dalian 116051,China, Dalian, Liaoning, 116044, CHINA.
⁵ Dalian 116051,China, Dalian Medical University, Dalian 116051,China, Dalian, Liaoning, 116044, CHINA.
⁶ General Hospital of Northern Theater Command, Shenyang 110016, China;, Shenyang, Liaoning, 110016, CHINA.
⁷ Northeastern University, Shenyang 110819, China, Shenyang, Liaoning, 110819, CHINA.
⁸ Key Laboratory for Anisotropy and Texture of Materials (Ministry of Education), Northeastern University, Shenyang 110004, Shenyang, Liaoning, 110819, CHINA.

PMID: 39612571
DOI: 10.1088/1748-605X/ad98d7

Abstract

The ability of osseointegration of implants is an important factor in ensuring the long-term stability of bone implants in their recipient sites. In this paper, Ti-M titanium alloys with different surface micro-area potential difference (MAPD) were prepared and the adhesion, proliferation, spreading, and differentiation behavior of osteoblasts (MC3T3) on the surface of Ti-M alloy were investigated in detail to reveal the effect of MAPD on cell compatibility and osteogenic differentiation. The results showed that the alloy with high MAPD exhibited facilitated bone differentiation, demonstrating that MAPD significantly enhanced the alkaline phosphatase activity and mineralization ability of osteoblasts, and upregulated the expression of osteogenic differentiation-related factors. It is suggested that it might be a strategy to promote the surface bioactivity of titanium alloy by adjusting the surface MAPD.&#xD.

Keywords: osteogenic differentiation; surface micro-area potential difference (MAPD); titanium alloys; titanium alloys; surface micro-area potential difference (MAPD); osteogenic differentiation;.