Combined effect of polystyrene nanoparticles and chlorpyrifos to Daphnia magna

Abstract

Microplastics and nanoplastics (MPs/NPs) are emerging contaminants ubiquitous in the environment. These particles can act as carriers of hydrophobic organic compounds (HOCs), such as chlorpyrifos (CPF), an organophosphorus insecticide. This study investigates the acute toxicity of CPF combined with model polystyrene nanoplastics (PS-NPs) using Daphnia magna as a model organism. The uptake and accumulation of luminescence-labeled 240 nm PS-NPs were visualized and quantified during co-exposure tests. Heart rates were measured using a video-based method, and IC₅₀ values for heart rate were calculated to assess the acute toxicity of CPF. The results demonstrate that PS-NPs alone have no acute toxicity, while CPF exhibits high toxicity, with an IC₅₀ of 50.8 μg/L. However, in the presence of 50 μg/L PS-NPs, the IC₅₀ increased to approximately 400 μg/L, indicating an antagonistic effect of PS-NPs on CPF toxicity. The adsorption of CPF onto PS-NPs can reduce the concentration of free CPF, leading to aggregation and sedimentation of CPF-PS-NP clusters, thereby decreasing the uptake of CPF by D. magna. This interaction between CPF and PS-NPs in D. magna exemplifies the toxicity-attenuating effects and potential load capacity of nanoplastics for HOCs.

Keywords: Chlorpyrifos; Combined toxicity effect; Polystyrene Nanoplastics.