Accurate Prediction of Protein-Binding Residues in Protein Sequences Using SCRIBER

Abstract

Deciphering molecular-level mechanisms that govern protein-protein interactions (PPIs) relies in part on the accurate prediction of protein-binding partners and protein-binding residues. These predictions can be used to support a wide spectrum of applications that include development of PPI networks and protein docking programs, drug design studies, and investigations of molecular details that underlie certain diseases. Computational methods that predict protein-binding residues offer convenient, inexpensive, and relatively accurate data that can aid these efforts. We introduce and describe a user-friendly webserver for the SCRIBER method that conveniently provides state-of-the-art predictions of protein-binding residues and that minimizes cross-predictions, i.e., incorrect prediction of residues that bind other/non-protein ligands as protein binding. SCRIBER relies on a two-layer architecture that is specifically designed to reduce the cross-predictions. We motivate and explain this predictive architecture. We describe how to use the webserver, interact with its web interface, and collect, read, and understand results generated by SCRIBER. The SCRIBER webserver is available at http://biomine.cs.vcu.edu/servers/SCRIBER/ .

Keywords: Cross-prediction; Logistic regression; Machine learning; Prediction; Protein-binding residues; Protein-protein interactions; SCRIBER; Web server.