Hypoglycemic effects of a new heteropolysaccharide from common bean (Phaseolus vulgaris L.) seeds in type 2 diabetes mellitus mice via modulating gut microbiota

Yaning Qu; Mengyue Xu; Fahu Yuan; Hongxing Zhang; Hui Li; Rui Guo; Jinyi Yu; Qinai Ren; Runkui Wang; Peng Wang; Hongbo Wang

doi:10.1016/j.ijbiomac.2024.137825

Hypoglycemic effects of a new heteropolysaccharide from common bean (Phaseolus vulgaris L.) seeds in type 2 diabetes mellitus mice via modulating gut microbiota

Int J Biol Macromol. 2024 Nov 19;283(Pt 3):137825. doi: 10.1016/j.ijbiomac.2024.137825. Online ahead of print.

Authors

Yaning Qu¹, Mengyue Xu¹, Fahu Yuan¹, Hongxing Zhang¹, Hui Li¹, Rui Guo², Jinyi Yu¹, Qinai Ren³, Runkui Wang³, Peng Wang³, Hongbo Wang⁴

Affiliations

¹ School of Life Sciences, Jianghan University, Wuhan 430056, China; Innovation Center for Comprehensive Utilization of Food and Medicine Homologous Specialty Resources, Wuhan 430056, China.
² School of Life Sciences, Jianghan University, Wuhan 430056, China; Hubei Province Engineering Research Center for Legume Plants, Wuhan 430056, Hubei, China.
³ School of Life Sciences, Jianghan University, Wuhan 430056, China.
⁴ School of Life Sciences, Jianghan University, Wuhan 430056, China; Innovation Center for Comprehensive Utilization of Food and Medicine Homologous Specialty Resources, Wuhan 430056, China; Hubei Province Engineering Research Center for Legume Plants, Wuhan 430056, Hubei, China. Electronic address: bobo110110165@sina.com.

PMID: 39571858
DOI: 10.1016/j.ijbiomac.2024.137825

Abstract

Type 2 diabetes poses significant health issues worldwide; however, relatively few effective treatment strategies are currently available. This research seeks to explore the potential hypoglycemic impact of compounds derived from common bean (Phaseolus vulgaris L.) by structurally characterizing a new type of heteropolysaccharide (CIE2-F) and evaluating its hypoglycemic effects in a murine model. CIE2-F primarily comprises 10 monosaccharides, Mw: 9.25 × 10⁵ Da. The polysaccharide exhibited significant anti-obesity effects, alleviated pathological liver damage, and reduced hyperglycemia. In addition, the polysaccharide mitigated insulin resistance and regulated dyslipidemia by increasing serum HDL-C and reducing LDL-C, total cholesterol, and triglycerides in diabetic mice. Furthermore, 16S rRNA sequencing revealed that CIE2-F enriched beneficial gut microbiota, including Akkermansia and Verrucomicrobia, while decreasing pathogenic bacteria.

Keywords: Common bean; Gut microbiota; Phaseolus vulgaris L.; Polysaccharide; Short-chain fatty acid; Structural characterization; Type 2 diabetes mellitus.