Utilization of edible poultry slaughter residues: A chicken-liver hydrolysate with glucose-lowering ability and upregulating glycogenesis in type II diabetes

Yi-Ling Lin; Yu-Pei Chen; Sheng-Yao Wang; Yi-Feng Kao; Chompunut Lumsangkul; Yi-Chen Chen

doi:10.1016/j.psj.2024.104517

Utilization of edible poultry slaughter residues: A chicken-liver hydrolysate with glucose-lowering ability and upregulating glycogenesis in type II diabetes

Poult Sci. 2024 Nov 7;104(1):104517. doi: 10.1016/j.psj.2024.104517. Online ahead of print.

Authors

Yi-Ling Lin¹, Yu-Pei Chen², Sheng-Yao Wang², Yi-Feng Kao³, Chompunut Lumsangkul⁴, Yi-Chen Chen⁵

Affiliations

¹ Department of Animal Science and Technology, National Taiwan University, Taipei City 106037, Taiwan; Undergraduate and Graduate Programs of Nutrition Science, National Taiwan Normal University, Taipei City 116059, Taiwan.
² Department of Animal Science and Technology, National Taiwan University, Taipei City 106037, Taiwan.
³ Seafood Technology Division, Fisheries Research Institute, Ministry of Agriculture, Keelung City 202008, Taiwan.
⁴ Department of Animal Science, National Chung-Hsing University, Taichung City 402202, Taiwan.
⁵ Department of Animal Science and Technology, National Taiwan University, Taipei City 106037, Taiwan; Master Program in Global Agriculture Technology and Genomic Science, International College, National Taiwan University, Taipei City 106319, Taiwan. Electronic address: ycpchen@ntu.edu.tw.

PMID: 39571203
DOI: 10.1016/j.psj.2024.104517

Abstract

Approximately 10,000 metric tons of broiler livers are yielded every year in Taiwan. However, due to unpleasant odor and health concern, these livers are typically discarded as waste in the slaughtering stream in most developed or developed countries. In alignment with global agrocycle policies, a biofunctional chicken-liver hydrolysate (CLH) has been developed. This study was to investigate the effects of CLHs on glucose homeostasis and complications in type II diabetes. Insulin resistance was induced in liver (FL83B) and muscle (C2C12) cells using 30 and 20 ng TNF-α/mL, respectively, resulting in decreased glucose uptake and lower expressions of IRβ, p-Akt/Akt, and p-GSK3/GSK. CLH supplementation significantly upregulated (p<0.05) glucose uptakes and these proteins. In db/db mice, CLH supplementation improved insulin resistance, as shown by OGTT assay, HOMA-IR value and serum glucose levels, while also reducing serum lipids and liver damage indices (p<0.05). Additionally, CLH ameliorated (p<0.05) decreased hindlimb-gastrocnemius weight, and liver lipid contents, oxidative stress (sera and liver) and inflammatory cytokines. Increased glycogen accumulation was visualized in PAS-stained liver and hindlimb tissues of db/db mice supplemented with CLHs, consistent with upregulated glycogenesis in TNF-α-induced liver and muscle cells through the IRβ-Akt-GSK3 pathway. These findings suggest CLH may offer a mitigation against hyperglycemia and associated complications in type II diabetes, while also highlighting a sustainable solution for utilizing poultry slaughter residues.

Keywords: Chicken-liver hydrolysate; Glycogenesis; In-vitro experiment; Type II diabetes; db/db mice.