SNHG12 in cancer-associated fibroblast-derived extracellular vesicle induces macrophage-myofibroblast transition

Tao Wu; Li Zeng; Chao Peng; Zheng Zhao; Weihao Mu; Song Wang; Deli Tan

doi:10.1080/17501911.2024.2430166

SNHG12 in cancer-associated fibroblast-derived extracellular vesicle induces macrophage-myofibroblast transition

Epigenomics. 2024 Nov 20:1-13. doi: 10.1080/17501911.2024.2430166. Online ahead of print.

Authors

Tao Wu¹, Li Zeng², Chao Peng³, Zheng Zhao³, Weihao Mu³, Song Wang³, Deli Tan³

Affiliations

¹ Cardiothoracic surgery department, Banan Hospital of Chongqing medical university, Chongqing, China.
² Department of Pathology, Chongqing Ninth People's Hospital, Chongqing, China.
³ Department of Thoracic surgery, Chongqing Ninth People's Hospital, Chongqing, China.

PMID: 39568326
DOI: 10.1080/17501911.2024.2430166

Abstract

Aims: To investigate mechanism of lncRNA SNHG12 induced macrophage-myofibroblast transition (MMT) in cancer-associated fibroblasts (CAFs)-derived extracellular vesicles (EVs) in non-small cell lung cancer (NSCLC).

Method: CAFs EVs were isolated from human NSCLC tissue and adjacent cancerous tissue (n = 3), and their morphology and particle size were evaluated. Macrophages and MMT cells with different phenotypes were detected, and the binding relationship of lncRNA SNHG12, miR-181a-5p, and Smad3 was verified.

Result: LncRNA SNHG12 derived from CAFs-EVs promoted the transformation of M2 macrophages into MMT. In addition, lncRNA-SNHG12 increased the expression of Smad3 which was significantly upregulated in MMT through sponge of miR-181a-5p.

Conclusion: LncRNA SNHG12 derived from CAFs-EV induced MMT in NSCLC.

Keywords: CAFs-EVs; MMT; Macrophages; NSCLC; Smad3; lncRNA SNHG12; miR-181a-5p.