Response of heterocyst differentiation of Dolichospermum to different forms of nitrogen deficiency

Abstract

In recent years, initiatives aimed at mitigating eutrophication have successfully reduced nitrogen and phosphorus concentrations in numerous lakes across China. Notably, the management of total nitrogen levels has prompted a shift in the dominant genera responsible for harmful algal blooms. Among these, Dolichospermum, a typical diazotrophic cyanobacterium, exhibits the ability to differentiate heterocysts for atmospheric N₂ fixation under nitrogen-limited conditions. However, the underlying mechanisms driving heterocyst differentiation in response to the absence of specific nitrogen compounds remain poorly understood. This study analyzed the driving factors influencing heterocyst frequency using field data from Lake Chaohu collected between January and June 2022. Furthermore, an experiment was conducted utilizing NH₄Cl, NaNO₃ and urea as nitrogen sources, with specific nitrogen deficiencies created to investigate the response mechanisms of Dolichospermum under these conditions. The results indicated significant monthly variations in heterocyst frequency in Lake Chaohu, which were associated with the interaction of multiple driving factors. Nutrient changes emerged as the most intuitive driving factor, with heterocyst frequency showing a significant negative correlation with total nitrogen and dissolved total nitrogen levels. Experimental results demonstrated that the absence of NO₃N promoted both the biomass and heterocyst frequency of Dolichospermum. When NH₄N was limited, the proliferation of Dolichospermum was inhibited, leading to an extended period of heterocyst development. Although a lack of urea eventually increased heterocyst frequency in Dolichospermum, there was no significant increase in biomass. The concentrations of the three nitrogen sources exhibited a negative correlation with heterocyst differentiation, with the effects of NO₃N and urea deficiency on heterocyst differentiation being significantly stronger than those of NH₄N. Moreover, heterocyst differentiation frequency was positively correlated with photosynthetic efficiency, which indicated that the acquisition and distribution of photosynthetic energy between heterocysts and vegetative cells also influence the differentiation process of heterocysts to some extent. The findings highlight the differing responses of heterocyst differentiation to various forms of nitrogen, emphasizing the importance of prioritizing NH₄N removal in nutrient control. However, further research is needed to determine the key threshold concentrations of different nitrogen sources that trigger heterocyst differentiation.

Keywords: Dolichospermum; Heterocyst differentiation; Nitrogen deficiency.