Fucoidan from Stichopus chloronotus relieved DSS induced ulcerative colitis through inhibiting intestinal barrier disruption and oxidative stress

Rui Li; Jiaojiao Mou; Lei Zhao; Mengqiu Hu; Bin Wang; Yanying Sun; Jing Liu; Xiaohui Qi; Jie Yang

doi:10.1016/j.ijbiomac.2024.137811

Fucoidan from Stichopus chloronotus relieved DSS induced ulcerative colitis through inhibiting intestinal barrier disruption and oxidative stress

Int J Biol Macromol. 2024 Nov 18:137811. doi: 10.1016/j.ijbiomac.2024.137811. Online ahead of print.

Authors

Rui Li¹, Jiaojiao Mou², Lei Zhao³, Mengqiu Hu², Bin Wang³, Yanying Sun², Jing Liu³, Xiaohui Qi⁴, Jie Yang⁵

Affiliations

¹ School of Pharmacy, Shandong Second Medical University, Weifang 261053, Shandong, China; College of Biological and Chemical Engineering, QiLu Institute of Technology, Jinan, Shandong, China.
² School of Public Health, Shandong Second Medical University, Weifang 261053, Shandong, China.
³ School of Pharmacy, Shandong Second Medical University, Weifang 261053, Shandong, China.
⁴ School of Life Science and Technology, Shandong Second Medical University, Weifang 261053, Shandong, China. Electronic address: qixh@sdsmu.edu.cn.
⁵ School of Pharmacy, Shandong Second Medical University, Weifang 261053, Shandong, China. Electronic address: yangjie@sdsmu.edu.cn.

PMID: 39566803
DOI: 10.1016/j.ijbiomac.2024.137811

Abstract

Intestinal barrier disruption and oxidative stress are the major pathological features during the ulcerative colitis (UC). The present research aimed to explore the amelioration property of fucoidan of Stichopus chloronotus (FucSc) against dextran sulfate sodium (DSS) resulted UC. The findings from our study suggest that treating with Fuc-Sc improved the integrity of the Caco-2 monolayer through raising its TEER value, reducing LDH release, promoting the tight junction proteins (TJs) in Caco-2 cells, and shielding the cells from decreases of these proteins caused by H₂O₂. Besides, Fuc-Sc activated Nrf2/HO-1 pathway to facilitate the antioxidant activities of Caco-2 cells under oxidative stress through elevating the SOD and GSH, and reducing LDH and MDA. Furthermore, oral administration of Fuc-Sc attenuated DSS resulted body weights decrease, DAI score increase, colon length decrease, and structural damage of colon tissue. Fuc-Sc also promoted the barrier function and suppressed oxidative injury via activation of Nrf2/HO-1 signal path. Collectively, this research provided the theoretical foundation for fucoidan as a promising functional food for colitis.

Keywords: Fucoidan; Intestinal barrier disruption; Nrf2/HO-1 pathway; Oxidative stress; Stichopus chloronotus.