Construction of a Dual-Mode Sensing Platform for Ultra-fast Detection of Bisulfite in Food and Environmental Systems

J Fluoresc. 2024 Nov 20. doi: 10.1007/s10895-024-04031-x. Online ahead of print.

Abstract

Sulfur dioxide (SO2) is widely used in food processing to extend the shelf life of food. However, excessive intake of SO2 and its derivatives (HSO3- and SO32-) can cause oxidative damage to the body, and result in several diseases. How to construct probes for rapid real-time detection of HSO3- in the field is beneficial to the developmental needs of practical applications, but it is also very challenging. Here we report a dual-mode fluorescent probe Rh-QL for ultrafast detection of HSO3-, which undergoes a specific 1,4-Michael addition reaction with HSO3- to achieve near-infrared fluorescence turn-on. Probe Rh-QL can rapidly (< 5 s) respond to HSO3- with a color change from purple to green and a strong fluorescence emission at 725 nm. The probe Rh-QL has been used for the detection of HSO3- in real sugar samples and can be prepared as a portable sensing kit for the detection of HSO3- in the environment due to its high efficiency, rapidity and accuracy. In addition, the probe Rh-QL is able to target label Gram-negative bacteria after reacting with HSO3-, which has the potential to identify the type of pathogenic bacteria.

Keywords: Bacteria targeting; Food detection; Near-infrared fluorescence; SO2 derivatives.